Methods for addressing the NK cell dysregulationWhat the fruck? First of all, when did the WPI use qRT-PCR? Why did they kept rather quiet about it? And secondly, this seems to have been done in the context of treatment? Were are the results of these studies?
…
- PBMCs from XMRV infected patients with low NK cell function were activated with the mitogen PHA and treated with Ampligen
- The effects on NK cell (CD56+) phenotype were determined by flow cytomentry
- Signaling changes due to the treatment were detected via cytokine analysis
- The change in XMRV copy number was detected with qRT-PCR.
Preliminary Results Cont.
XMRV copy number is modulated by Ampligen
When treated with Ampligen, qRT-PCR indicates a decrease in some patients and an increase in others.
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