Thursday, March 8, 2012

All. Cells.

For example, it is usual to find in any viral infection that infected and uninfected cells exist in equilibrium as two discrete populations. In flow cytometric analysis, this is represented by a bimodal peak. Flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) taken from CFS patients was shown in the paper as a single peak, indicating that almost every cell was infected with XMRV.

Let me translate:

In the Lombardi et al. 2009 study all cells tested by intracellular flow cytometry (IFC) showed reaction with the rat anti-MLV p30 Gag monoclonal antibody.

All cells reacted with the anti-MLV antibody.

All cells reacted.

All cells.

All. Cells.

All.

All of them.

Each and every one of these little buggers reacted with the anti-body.

All PBMCs reacted with the antibody.

B-cells.

T-cells.

NK-cells.

The whole shebang.

And not only is each type of PBMCs reacting, no, the 100% peak shift means that each cell of the PBMCs is reacting. Not a single one left that does not react.

Now, if this reaction is caused by an infectious agent, then this would mean that each and every cell would be infected.

Now if each cell would be infected, then the virus would be easy to find having the right PCR primers/probes.

No need to mess around with nested PCR, do a single round PCR and find it. Because it would be in each and every cell.

Now, Mikovits claims to have found XMRV before Christmas 2008. Ruscetti got involved shortly after that. They submitted the study in May 2009. Enough time since then to come up with the actual sequences, if it wasn't XMRV.

Yet, nothing. No sequences other than XMRV. In now over three years since Christmas 2008.
Posted by Tony Mach Posted on Thursday, March 08, 2012
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