Monday, February 27, 2012

XMRV VP62 plasmid was used in Lombardi et al. 2009

Another oldie but goodie: XMRV VP62 plasmid was used in Lombardi et al. 2009. And not only that, but it looks like VP62 was used to test their Western Blots – some people might call this "calibrating the assay to VP62".

From Lombardi et al. 2009:
All of these Abs detected the human VP62 XMRV strain grown in human Raji, LNCaP and Sup-T1 cells (fig. S3) (5).
Figure S3. Detection of cloned XMRV-VP62 using a rat mAb to SFFV Env and a goat antiserum to mouse NZB xenotropic MLV.

A. Lysates were prepared from XMRV-VP62-infected Raji (lane1), LNCaP (lane 2) or Sup-T1 (lane 3). Positive controls used were HCD-57 cells, a mouse erythroleukemia cell line expressing polytropic MLV gp70 Env (lane 4), and HCD-57 cells infected with SFFV, which also express SFFV gp55 Env (lane 5). WB analysis was carried out using rat anti-SFFV Env mAb 7C10. Molecular weight markers in kD are shown on the left.

B. Lysates were prepared from XMRV-VP62-infected Raji (lane 1), LNCaP (lane 2) or Sup-T1 (lane 3). Lysates from SFFV-infected mouse HCD-57 cells (lane 4) and from uninfected Raji, LNCaP and Sup-T1 are shown in lanes 5-7, respectively. WB was carried out using goat antiserum to mouse NZB xenotropic MLV. Molecular weight markers in kD are shown on the left.
As a note: Fig. S3 was probably exported as a JPG (complete with all text) or even scanned, and then imported as an image – no other figure in Lombardi 2009 was treated like this.

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