Saturday, January 28, 2012

How many of the 101 patient samples from Lombardi et al 2009 were positive for XMRV? 68? 99? 76? 67? 86?

Lombardi et al 2009 (submitted 14 July 2009, accepted 31 August 2009) reported 68 of the 101 patient samples to be positive:
Of the 101 CFS samples analyzed, 68 (67%) contained XMRV gag sequence.
So we have 67% positive samples by July 2009.

In the CFSAC October 2009 meeting, the WPI reported 99 out 101 samples to be positive:
Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients
19/33 Antibodies in the plasma
30/33 Transmissable virus in the plasma
10/33 Protein expression in Decitibine (5Aza2DC) treated PBMC

Thus, since the submission to Science we determined 99 of 101 US patients show evidence of XMRV infection
This would amount to 98% positive samples by October 2009.  Dr. Judy Mikovits has used this "fact" in many of her talks.

In the addendum to Lombardi et al. 2009 (Submitted 26th February 2010, revised 20th May 2010, accepted: 24th May 2010) this number goes suddenly down:
In that study, we used a combination of biological amplification and molecular enhancement techniques to detect XMRV in more than 75% of 101 patients with chronic fatigue syndrome (CFS).
So we are down to 75% positive samples by February/May 2010. And please note that no absolute number of positive samples is given, only the percentage is reported.

But it get better, in her "Dear Dr. Alberts" letter from May 30th 2011, Dr. Judy Mikovits forget everything that happened before and has even problems remembering the number correctly that was originally reported by Lombardi et al 2009.
Using four different methods including PCR (of cultured and co-cultured cells), detection of human gammaretroviral (HGRV) viral proteins (culture and co-culture detected by Western Blot and flow cytometry), anti-gammaretrovirus Env antibodies in human serum (competed by 7C10 rat monoclonal antibody), and virus isolation from primary cell and co-cultures, we reported evidence of human gammaretrovirus infection in at least 67 out of 101 CFS patients.
Now we are down to "at least" 66% positive samples in May 2011.

And now we have at least 67 out of 101, when Lombardi reported 68 out of 101, Mikovits said in her talks 99 out of 101 and the addendum says 76 out of 101.

Hmm.

So how many patient samples were positive?

68?

99?

76?

67?

Update:
Or is it 86 out of 101? (or 85% as reported)

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