Upon joining WPI July 1, 2007, Dr. Lombardi's research effort focused on the inflammatory component of CFS and its relationship to the development of lymphoma in CFS patients. It was at this time that Dr. Lombardi established his collaboration with Robert Silverman, Ph.D., of the Cleveland Clinic, the world's leading authority regarding the RNase L antiviral pathway. Dr. Silverman had recently made the discovery of XMRV in RNase L deficient cancer patients. Dr. Lombardi's research on the association of the RNase L pathway in CFS patients, and discussions with Dr. Silverman prompted him to begin the search for XMRV in CFS.Mikovits first contact with XMRV:
In October 2007, Mikovits attended a prostate-cancer meeting near Lake Tahoe, Nevada, where she met Robert Silverman, a virologist at the Cleveland Clinic in Ohio. …Reagents from Silverman:
At the meeting, Silverman was presenting research linking XMRV to deficiencies in a virus-defence pathway.
In March 2008 Dr. Mikovits got XMRV VP62 plasmid from Dr. Silverman.First positive samples:
Just before Christmas 2008, Mikovits turned her attention to Silverman's reagents. She and her postdoc, Vincent Lombardi, known as Vinny, asked a graduate student to test for XMRV DNA in white blood cells [by PCR?] from some of the most seriously ill people being studied at the WPI.Involvement of Dr. Ruscetti:
The first try turned up just two positives out of 20. But by tweaking the conditions of the test, Mikovits says her team found XMRV in all 20.
The WPI has examined all 218 control and 101 patient samples by both PCR and serological methods for the presence of XMRV nucleic acid and antibodies. In addition, NCI used plasma from all 100 samples they received in infection experiments with LNCaP cells.Submission of Lombardi et al. 2009:
[Response to Comments on “Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome”, Mikovits and Ruscetti]
At what date got Dr. Ruscetti's lab involved? Must have been after Christmas 2008, according to this timeline.
In May 2009, the team submitted a paper to Science reporting the identification of XMRV genetic material in two-thirds of the 101 patients with chronic fatigue they had tested and in 3.7% of 218 healthy people.Submission of the Addendum:
Sometime before June 2010 [http://parakoch.blogspot.com/2012/02/its-worse-than-i-thought.html]